|
|
|
|
TimRuns
Member
|
|
|
Joined: 11 May 2003
Posts: 10062
Location: Coquitlam, British Columbia
|
| Back to top
|
|
 Posted: 11/03/03 - 22:35 Post subject: Poor Froggie
Today in physiology lab we had to kill and decapitate a live frog for an experiment on action potentials and nerve impulses. We had to dissect out a nerve from the leg and conduct the experiments on it The TA placed the frog in a tub of 10% ethanol (alcohol) and it got drunk and pass out. She then decapitated it with a mini "guillotine". Poor froggie....wait till crazyfrog reads this 
|
|
|
|
|
airehead
Oompa Loofah
|
|
|
Joined: 12 Nov 2002
Posts: 18788
Location: Between here and eternity...
|
| Back to top
|
|
Posted: 11/03/03 - 22:40 Post subject:
Yikes! Was this before or after lunch? 
You must be pretty dedicated--although it is for learning, I would still find it a little difficult to watch.
|
|
|
|
|
TimRuns
Member
|
|
|
Joined: 11 May 2003
Posts: 10062
Location: Coquitlam, British Columbia
|
| Back to top
|
|
Posted: 11/03/03 - 22:44 Post subject:
after lunch fortunately but about 2.5 hours before dinner..The decapitation part was the worst part to watch...
|
|
|
|
|
omega lambda
Member
|
|
|
Joined: 14 May 2002
Posts: 4691
|
| Back to top
|
|
Posted: 11/03/03 - 22:51 Post subject:
Egads!
Did you have to have a fresh dead frog for the experiment? Kinda sucks that you had to do that.
|
|
|
|
|
TimRuns
Member
|
|
|
Joined: 11 May 2003
Posts: 10062
Location: Coquitlam, British Columbia
|
| Back to top
|
|
Posted: 11/03/03 - 22:53 Post subject:
| omega lambda wrote: | Egads!
Did you have to have a fresh dead frog for the experiment? Kinda sucks that you had to do that. |
of course! We had the get a fresh nerve to do our experiments on (to be able to stimulate it with electrodes and get an action potential)
|
|
|
|
|
omega lambda
Member
|
|
|
Joined: 14 May 2002
Posts: 4691
|
| Back to top
|
|
Posted: 11/03/03 - 23:14 Post subject:
| TimRuns wrote: | | omega lambda wrote: | Egads!
Did you have to have a fresh dead frog for the experiment? Kinda sucks that you had to do that. |
of course! We had the get a fresh nerve to do our experiments on (to be able to stimulate it with electrodes and get an action potential) |
Yeah, that's what I was thinking. I was just wondering how much time you have to work with the frog, so that maybe "they" could give you an already dead frog to work on. And I guess the next question is, why is it so important to measure the potentials yourself? I think I'd be very happy to just know what someone else discovered in this case. 
|
|
|
|
|
crazyfrog
Kermitologist
|
|
|
Joined: 31 May 2003
Posts: 7610
Location: atlanta
|
| Back to top
|
|
Posted: 11/04/03 - 00:40 Post subject:
have you ever seen a hamster knocked out and then decapitated with that mini guillotine?
well that was what was going on behind me in the lab where i was slicing fish heads today. the techs were perfusing hamsters, which basically means that the hamsters were being fixed from the inside out by anesthestizing them, cutting thier hearts open and pushing out all of the blood and pushing in paraformaldehyde in its place. once the cute little cuddilys are stiff, their heads are chopped off and thier brains disected.
hows this for gruesom? there were blood splatters on the side of the hood where the "operations" were taking place....
for my research and the other projects going on on my lab, i have prolly sacrificed dozens of fish and its never phased me. but when i see cute, unsuspecting hamsters getting whacked, it kinda tugs at me abit. strange how some things in science bother you and others dont... 
|
|
|
|
|
TimRuns
Member
|
|
|
Joined: 11 May 2003
Posts: 10062
Location: Coquitlam, British Columbia
|
| Back to top
|
|
Posted: 11/04/03 - 01:46 Post subject:
| omega lambda wrote: | | TimRuns wrote: | | omega lambda wrote: | Egads!
Did you have to have a fresh dead frog for the experiment? Kinda sucks that you had to do that. |
of course! We had the get a fresh nerve to do our experiments on (to be able to stimulate it with electrodes and get an action potential) |
Yeah, that's what I was thinking. I was just wondering how much time you have to work with the frog, so that maybe "they" could give you an already dead frog to work on. And I guess the next question is, why is it so important to measure the potentials yourself? I think I'd be very happy to just know what someone else discovered in this case. |
We were given 4 hours to work on it-that was just enough time for us to work on it considering that we screwed up once on damaging 1 nerve. The main purpose of this experiment is to study the properties of the action potential and yup we will be given additional references on previous experiments done on this in order to help explain our results and write up the report.
|
|
|
|
|
TimRuns
Member
|
|
|
Joined: 11 May 2003
Posts: 10062
Location: Coquitlam, British Columbia
|
| Back to top
|
|
Posted: 11/04/03 - 01:57 Post subject:
| crazyfrog wrote: | have you ever seen a hamster knocked out and then decapitated with that mini guillotine?
well that was what was going on behind me in the lab where i was slicing fish heads today. the techs were perfusing hamsters, which basically means that the hamsters were being fixed from the inside out by anesthestizing them, cutting thier hearts open and pushing out all of the blood and pushing in paraformaldehyde in its place. once the cute little cuddilys are stiff, their heads are chopped off and thier brains disected.
hows this for gruesom? there were blood splatters on the side of the hood where the "operations" were taking place....
for my research and the other projects going on on my lab, i have prolly sacrificed dozens of fish and its never phased me. but when i see cute, unsuspecting hamsters getting whacked, it kinda tugs at me abit. strange how some things in science bother you and others dont... |
I agree with you on that point. I probably killed thousands of bugs in bioassays last summer but I hardly felt bad about it. On the other hand there was another scientist working in the same lab on rats. He basically fed the rats with this experimental diet and killed them by gassing them.He then dissected the rats and looked at how the diet was affecting their organs and growth. I guess it depends on what types of critters you are working on.
|
|
|
|
